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PCR—the polymerase chain reaction—is a crucial tool. The DNA1 amplification2 technique is used in genome sequencing, forensics and the diagnosis3 of various diseases.
To give researchers more genetic4 material to work with, a PCR instrument repeatedly heats and cools an original biological sample. Which gives enzymes5 a chance to replicate6 the DNA millions of times so it can be more easily analyzed7.
Such sequence amplification would be a boon8 to diagnosis in a doctor's office—especially when an infectious disease is spreading rapidly. Unfortunately, genetic tests usually take a day or two to complete. But researchers at the Lawrence Livermore National Laboratory aimed to speed up the process.
First they created an extremely fast thermal9 cycle, in which a sample experiences 45 degrees Celsius10 temperature changes per second. Then they searched for DNA amplification enzymes that could operate at that thermal cycling speed. And they found two that worked without any tweaking. The technique accomplishes a billion-fold amplification in well under three minutes. The work is in the journal Analyst11.
The system could make it possible to identify food contamination virtually instantly. Or an infection before you even finish coughing.
Thanks for the minute. For Scientific American's 60-Second Science, I'm Cynthia Graber.
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1 DNA | |
(缩)deoxyribonucleic acid 脱氧核糖核酸 | |
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2 amplification | |
n.扩大,发挥 | |
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3 diagnosis | |
n.诊断,诊断结果,调查分析,判断 | |
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4 genetic | |
adj.遗传的,遗传学的 | |
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5 enzymes | |
n. 酶,酵素 | |
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6 replicate | |
v.折叠,复制,模写;n.同样的样品;adj.转折的 | |
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7 analyzed | |
v.分析( analyze的过去式和过去分词 );分解;解释;对…进行心理分析 | |
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8 boon | |
n.恩赐,恩物,恩惠 | |
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9 thermal | |
adj.热的,由热造成的;保暖的 | |
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10 Celsius | |
adj.摄氏温度计的,摄氏的 | |
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11 analyst | |
n.分析家,化验员;心理分析学家 | |
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